BioRun Seamless Clone Mix Kit在线工具
单酶切线性化
双酶切线性化
IIs 酶线性化
PCR扩增线性化
DNA使用量计算
请选择载体
pET-30a
pET30a-6xHis
pBWRA(V)H32ib
LALR
LBLR
pBWA(V)HS-ccdB
pBWA(V)HU-ccdB
pBWA(V)KS-ccDB
pBWA(V)KU-ccdB
pBWD(LA)C
pBWD(LB)C
pBWRD(LB)1C-CCDB
pBWD(LB)1C-CCDB
pBWA(V)HS-ccdb-osgfp
pBWA(V)HS-ccdb-GLosgfp
重组后是否保留酶切位点:
否
是
请选择酶切位点,5'至3'方向依次选择
AvrII [CCTAGG]
BamHI [GGATCC]
BglII [AGATCT]
EcoRI [GAATTC]
HindIII [AAGCTT]
KpnI [GGTACC]
NcoI [CCATGG]
NdeI [CATATG]
NheI/BmtI [GCTAGC]
PacI [AATTAA]
PstI [CTGCAG]
SacI [GAGCTC]
ScaI [AGTACT]
SmaI/XmaI/TspMI [CCCGGG]
SpeI [ACTAGT]
SphI [GCATGC]
XbaI [TCTAGA]
XhoI [CTCGAG]
AclI [AACGTT]
ApaI [GGGCCC]
ApaLI [GTGCAC]
AscI [GGCGCGCC]
AsiSI [GCGATCGC]
AseI [ATTAAT]
BclI [TGATCA]
BglII [AGATCT]
BmgBI [CACGTC]
ClaI/BspDI [ATCGAT]
BstBI [TTCGAA]
BsrGI [TGTACA]
BssHII [GCGCGC]
BssSI [CACGAG]
DraI [TTTAAA]
EagI [CGGCCG]
FseI [GGCCGGCC]
FspI [TGCGCA]
HpaI [GTTAAC]
MfeI [CAATTG]
MluI [ACGCGT]
MscI [TGGCCA]
NaeI [GCCGGC]
NarI/PluTI [GGCGCC]
NotI [GCGGCCGC]
NruI [TCGCGA]
NsiI [ATGCAT]
PacI [TTAATTAA]
PciI [ACATGT]
PmeI [GTTTAAAC]
PmlI [CACGTG]
PsiI [TTATAA]
PvuI [CGATCG]
PvuII [CAGCTG]
SacII [CCGCGG]
SalI [GTCGAC]
SbfI/SdaI [CCTGCAGG]
SspI [AATATT]
StuI [AGGCCT]
SwaI [ATTTAAAT]
ZraI [GACGTC]
AvrII [CCTAGG]
BamHI [GGATCC]
BglII [AGATCT]
EcoRI [GAATTC]
HindIII [AAGCTT]
KpnI [GGTACC]
NcoI [CCATGG]
NdeI [CATATG]
NheI/BmtI [GCTAGC]
PacI [AATTAA]
PstI [CTGCAG]
SacI [GAGCTC]
ScaI [AGTACT]
SmaI/XmaI/TspMI [CCCGGG]
SpeI [ACTAGT]
SphI [GCATGC]
XbaI [TCTAGA]
XhoI [CTCGAG]
AclI [AACGTT]
ApaI [GGGCCC]
ApaLI [GTGCAC]
AscI [GGCGCGCC]
AsiSI [GCGATCGC]
AseI [ATTAAT]
BclI [TGATCA]
BglII [AGATCT]
BmgBI [CACGTC]
ClaI/BspDI [ATCGAT]
BstBI [TTCGAA]
BsrGI [TGTACA]
BssHII [GCGCGC]
BssSI [CACGAG]
DraI [TTTAAA]
EagI [CGGCCG]
FseI [GGCCGGCC]
FspI [TGCGCA]
HpaI [GTTAAC]
MfeI [CAATTG]
MluI [ACGCGT]
MscI [TGGCCA]
NaeI [GCCGGC]
NarI/PluTI [GGCGCC]
NotI [GCGGCCGC]
NruI [TCGCGA]
NsiI [ATGCAT]
PacI [TTAATTAA]
PciI [ACATGT]
PmeI [GTTTAAAC]
PmlI [CACGTG]
PsiI [TTATAA]
PvuI [CGATCG]
PvuII [CAGCTG]
SacII [CCGCGG]
SalI [GTCGAC]
SbfI/SdaI [CCTGCAGG]
SspI [AATATT]
StuI [AGGCCT]
SwaI [ATTTAAAT]
ZraI [GACGTC]
同源臂长度
bp
     引物和片段结合长度
bp
插入片段个数:
1
2
3
4
5
6
请输入每个插入DNA片段: (序列默认为5'至3'方向)
生成引物
生成载体序列
清空