BioRun Seamless Clone Mix Kit在线工具
单酶切线性化
双酶切线性化
IIs 酶线性化
PCR扩增线性化
DNA使用量计算
请选择载体
pET-30a
pET30a-6xHis
pBWRA(V)H32ib
LALR
LBLR
pBWA(V)HS-ccdB
pBWA(V)HU-ccdB
pBWA(V)KS-ccDB
pBWA(V)KU-ccdB
pBWD(LA)C
pBWD(LB)C
pBWRD(LB)1C-CCDB
pBWD(LB)1C-CCDB
pBWA(V)HS-ccdb-osgfp
pBWA(V)HS-ccdb-GLosgfp
请选择酶切位点,5'至3'方向依次选择
BsaI/Eco31I- [NGAGACC]
SapI/LguI-/BspQI- [NGAAGAGC]
BsmBI/Esp3I- [NGAGACG]
BbsI/BpiI- [NNGTCTTC]
AarI- [NNNNGCAGGTG]
BveI-/Lsp1109I-/BfuAI-/BspMI- [NNNNGCAGGT]
AlwI- [NNNNGATCC]
Alw26I-/BcoDI-/BsmAI- [NGAGAC]
BccI- [NNNNGATGG]
BmrI- [NNNNCCCAGT]
BsrDI- [NNCATTGC]
BtsI- [CACTGC]
EarI- [NGAAGAG]
FokI- [NNNNNNNNNCATCC]
BbvCI- [GCTGAGG]
I-SceI+ [TAGGGATAACAGGGTAAT]
I-CeuI- [TTCGCTACCTTAGGACCGTTATAGTTA]
PI-PspI+ [TGGCAAACAGCTATTATGGGTATTATGGGT]
PI-SceI- [CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT]
Rare Cut I- [CGAGACGTCATCACGTTACACCTAAA]
Rare Cut II- [TGAGACCCTGAATCTCGTCCTCGAAA]
Rare Cut III- [CGAAGAGCTCGTTAGATGCTGCGAAA]
AscI [GGCGCGCC]
AsiSI [GCGATCGC]
FseI [GGCCGGCC]
NotI [GCGGCCGC]
PacI [TTAATTAA]
PmeI [GTTTAAAC]
SbfI/SdaI [CCTGCAGG]
SwaI [ATTTAAAT]
BsaI/Eco31I+ [GGTCTCN]
SapI/LguI+/BspQI+ [GCTCTTCN]
BsmBI/Esp3I+ [CGTCTCN]
BbsI/BpiI+ [GTCTTCNN]
AarI+ [CACCTGCNNNN]
BveI+/Lsp1109I+/BfuAI+/BspMI+ [ACCTGCNNNN]
AlwI+ [GGATCNNNN]
Alw26I+/BcoDI+/BsmAI+ [GTCTCN]
BccI+ [CCATCNNNN]
BmrI+ [ACTGGGNNNN]
BsrDI+ [GCAATGNN]
BtsI+ [GCAGTG]
EarI+ [CTCTTCN]
FokI+ [GGATGNNNNNNNNN]
BbvCI+ [CCTCAGC]
I-SceI- [ATTACCCTGTTATCCCTA]
I-CeuI+ [TAACTATAACGGTCCTAAGGTAGCGAA]
PI-PspI- [ACCCATAATACCCATAATAGCTGTTTGCCA]
PI-SceI+ [ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG]
Rare Cut I+ [TTTAGGTGTAACGTGATGACGTCTCG]
Rare Cut II+ [TTTCGAGGACGAGATTCAGGGTCTCA]
Rare Cut III+ [TTTCGCAGCATCTAACGAGCTCTTCG]
AscI [GGCGCGCC]
AsiSI [GCGATCGC]
FseI [GGCCGGCC]
NotI [GCGGCCGC]
PacI [TTAATTAA]
PmeI [GTTTAAAC]
SbfI/SdaI [CCTGCAGG]
SwaI [ATTTAAAT]
同源臂长度
bp
     引物和片段结合长度
bp
插入片段个数:
1
2
3
4
5
6
请输入每个插入DNA片段: (序列默认为5'至3'方向)
生成引物
生成载体序列
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